pET-32a plasmid

pET-32a is a commonly used prokaryotic expression vector of fusion protein type, which contains ampicillin resistance gene. Expression is induced by T7 RNA polymerase provided by the host cell.

pET-32a is recommended to clone E.coli DH5a or TOP10 as the recipient bacteria; use E.coli BL21 (DE3) or BL21 (DE3) pLysS as the recipient bacteria for expression.